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However, qPCR using EG tolerated a significantly higher dye concentration, thus permitting a more robust PCR signal as well as a sharper and stronger DNA melt peak.
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Low levels of contamination with maternal blood in the amniotic fluid are tolerated by the more robust PCR-SSP method.
ABC-PCR was more robust against the PCR amplification inhibitors than real-time PCR.
In this context, competitive PCR is more robust than real-time PCR, although its application to a large-scale analysis had been hampered by the need to prepare a competitor standard for each target; a target and its competitor standard have to be co-amplified with a single primer pair to give products that differ in size.
The microarray procedure alone was not expected to comprehensively identify the complete S. aureus essential gene list, but was applied as a screen to reduce the workload for the more robust but laborious PCR-based analysis.
Although not globally improved in all patients treated with T + C, anti-HER2 CD4+ T-cell immunity is more robust in patients achieving pCR compared with their non-pCR counterparts despite controlling for relevant demographic and tumor-related confounders.
The next-generation droplet digital polymerase chain reaction (ddPCR) assay employs an emulsion-based endpoint to quantitate the amount of target DNA and is more robust than real-time PCR when analyzing sequence variations.
The amplification efficiency of NASBA is more robust than that of PCR (Mercier-Delarue et al. 2014), yielding more than 1012 amplicons in as little as 30 min (Zhao et al. 2009).
The stability of the target RNA used for RT-PCR is more robust than that of viable virus because degradation of any part of the genome (or proteins and lipids) would compromise the ability of the virus to replicate.
To better mesh with the capabilities of newest-generation STR analysis assays, the Quantifiler® HP and Quantifiler® Trio DNA Quantification Kits were designed for greater detection sensitivity and more robust performance with samples that contain PCR inhibitors or degraded DNA.
Real-time PCR has proved more robust than ddPCR and can also be performed on DNA extracted from formalin-fixed paraffin-embedded tissue sections [ 14, 15].
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