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While these methods have allowed many successful studies of RNA protein interactions, challenges still exist for more robust assays.
More robust assays that cover a broad range of ROS (e.g. the DHE tissue stain, nitro-tyrosine detection, etc).
The first gap-PCR assays were subject to technical failure through ADO, but the more recently published primers and conditions support more robust assays.
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With an easier and more robust assay format than CA 15.3, EMCA is potentially a more useful marker.
Our much larger study with a more robust assay demonstrates that ADM levels on admission were strongly associated with the 28-day mortality rate.
For instance, in the omics technology arena, either changes in DNA copy number measured by microarray-based comparative genomic hybridization or patterns of peptide expression measured by mass spectrometry may eventually provide similar information in a more robust assay than expression profiling [ 6, 23].
However, a more robust viability assay would be needed to confirm these assumptions, such as testing for expression of a fluorescently labeled protein in real time.
Thus, there is a need for a more robust genotyping assay that is fast, sensitive, and can be applied directly to processed sputum samples without prior culturing.
DNA-based assays are often more robust than RNA-based assays, and whereas the mutation spectrum causing TSG inactivation is usually diverse (which limits the utility of mutation-specific detection strategies for tumor screening programs), TSG inactivation by promoter hypermethylation provides a more homogeneous target for molecular screening strategies.
Removing EBV from the CMV immune response assay could conceivably reduce both technical and biological variability in the response profile, resulting in a more robust and informative assay.
By extending the measurement, the investigation will be more robust to intra-assay variations of refluxes over time.
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