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Interestingly, we consistently observed a more robust amplification of the GA-repeat region than the GC-rich region in our ChIP assays (Fig. 3B and C), which may indicate the presence of more EWS/WT1 in the GA-repeat region (which contains more than 50 EWS/WT1(+KTS) binding sites).
During protocol optimization, we found that a gel-based size selection step led to more robust amplification of the desired size fraction.
Therefore our studies have focused on understanding the signals that regulate these processes towards achieving a more robust amplification of the stem/progenitor cell pool.
Therefore our most recent studies have focused on understanding the signals that regulate these processes towards achieving a more robust amplification of the stem/progenitor cell pool and more significant repair.
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Our results point to a more robust biological amplification process that potentially provides a common second messenger for many of garlic's diverse physiological effects.
These defects suggest that Tej and Tap together could support primary processing, probably by engaging Aub and/or Piwi, and a more robust ping-pong amplification cycle for piRNA amplification.
This approach is more robust, since the amplification efficiency of different amplicons is taken into account.
ABC-PCR was more robust against the PCR amplification inhibitors than real-time PCR.
However, amplification was more robust using high and low copy vectors as template.
However, ELF3 was one of the more robust markers giving very strong amplification of single tumour cells at 45 cycles.
In all cases, actin amplifications were more robust for tissue templates, reflecting higher template quantities in the parallel control reactions.
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