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The dissimilarity matrix was generated twice for pocket vs. pocket using a similar but more rigorous threshold, by expanding the binding pocket vicinity atoms to 6.5 Å, which should constitute a more difficult case than selecting a cut-off of 4 or 5 Å.
Both were subsequently given a more rigorous threshold detection evaluation.
Likewise, a more rigorous threshold was applied by using the flag IsWellAboveBG, which first determines if the feature is significant (IsPosAndSignif) and then determines if the background-subtracted signal is greater than 2.6 times the background standard deviation for that feature (approximates a 99% CI).
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During this second round of searching, homologous fragments are distinguished from possible false positive fragments by using a more rigorous expect value threshold (< e-10).
Unlike the Canberra distance, similarity function χ 2 was more rigorous in most thresholds than the ComplOverlap methodology.
In the absence of longer-term data from which to establish more rigorous baselines, we defined a threshold for WS outbreaks across all locations by statistically isolating unusually high disease events.
For a more rigorous mathematical reasoning of this threshold choice, see [ 20].
The proposed index has been shown to be more rigorous because it uses quality thresholds from the Quebec IQBP index (reputed to be very stringent), but yet it conserves the expert knowledge embodied in the Moroccan IMBP index.
However, this result is not robust to change in detection threshold, or when tested using more rigorous statistical analyses such as the Kolmogorov Smirnov test.
This approach uses several arbitrary thresholds (e.g. 300 °C for the maximum temperature composite image, Fig. 2c) based on rough trial and error, and these thresholds could be improved with a more rigorous optimization.
Values close to clinical thresholds could be repeated or a more rigorous stimulated test (mixed-meal or glucagon tests) performed.
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