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In the case that the region-of-interrogation contains multiple layers of tissue, a more rigorous expression is found by combining Eqs.
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However, the original cMonkey implementation instead used a probabilistic model that enabled a more rigorous integration of co-expression with bicluster evidence based on non-gene expression data types [ 3].
Given the inherent variability of expression arrays [ 13], combining both techniques for the analysis of microscopic biopsies may offer a more rigorous framework for preliminary analysis of expression data [ 14].
Secondly, I conducted principal components analysis to investigate the relationship between gene structure and expression in a more rigorous way.
However, as new generation oligomer chemistries were developed and experimental design was refined and became more rigorous, additional methodologies to manipulate gene expression using AOs were developed, including translation suppression, gene silencing and modification of pre-mRNA splicing (for review see Bennett and Swayze 83).
Since miRNAs played important regulatory roles by targeting mRNAs for cleavage or translational repression, the higher copy number of the conserved miRNA species in T. flavidus indicated that its gene expressions were under more rigorous monitoring.
The expression of SSEA3, a more rigorous pluripotency cell surface marker, was increased and became more homogeneous after NANOG elevation (Figs. 5G and S3).
The approach allows us to assess the quality of the expression data and is more rigorous than spot-checking a random collection of genes.
A larger study will allow for a more rigorous statistical analysis to assess differential miRNA expression.
A more rigorous test of the contribution of translational selection to gene expression is whether expression level can be predicted for individual genes exclusively based on codon usage.
Their films were marked by greater artistic self-expression and a somewhat more rigorous historical realism.
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