While we could replicate these results, we detected very little fusion with the more rigorous assay of lumenal compartment mixing.
Moving to a more resistant substrate makes for a more rigorous assay but even cotton contains a little amorphous cellulose.
Therefore, oxidative DNA damage could be higher in the WBCs of workers highly exposed to asbestos fibers but more rigorous assay methodology will be required for validation.
We reported that WRN depletion by shRNA increased cellular sensitivity to Cr VI) by a cell viability assay [11], and confirmed this result here using the more rigorous clonogenic assay.
Although evidence that the system might work in human cells was presented, it needs further validation in more rigorous pluripotent assays [10], [11].
Recently, incurred (patient) sample reanalysis (ISR) has been strongly recommended in bioanalysis as a more rigorous test of assay reproducibility, rather than the use of QCs (Fast et al, 2009).
To this aim, more rigorous pull-down assays have been designed, as immunoprecipitation of labelled miRNA/mRNA complexes and consequent target identification by RT-PCR and sequencing (Hsu et al, 2009), or immunoprecipitation with Ago2 antibody, thus isolating the ternary, presumably functional, miRNA/mRNA/Ago2 complex (Chi et al, 2009).
Thus even with a more rigorous tumor cell survival assay where any surviving cells had several days to recover and grow in the absence of drug, FADD-DD provides no protection against etoposide-induced death, while providing complete protection against TRAIL.
For more rigorous validation and testing they were tested again by the IP microarray assay in duplicate, providing 2 additional replicates of each.
Such observations are encouraging and warrant further and more rigorous studies to assess potential adverse effects on the embryos caused by OET assay.
In a more rigorous investigation of the in vitro protein-protein interaction between Apm1 and Rho3, we performed an assay using affinity capillary electrophoresis [16], [17] (Materials and Methods).
