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It should be noted that in several proteins we identified, but did not catalog, one or more sequence that conformed to the MAPK-docking motif of Bardwell and colleagues but did not conform to the more restrictive sequence that we used for a D-domain.
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Though potentially more restrictive and biased than classical shotgun sequencing approaches, functional metagenomics is advantageous, because it drastically reduces the volume of sequence analysis that is involved and considerably increases the quantity of information relating to a targeted family of functions.
Detailed analyses of single genes and gene clusters as well as large sequence polymorphisms (LSPs) and phylogenetic relationships were performed by more restrictive parameters (E value ≤ 10 − 20, alignment length ≥ 95% of query, sequence similarity ≥ 90%; depending on the kind of analysis) and manual investigation of all BLAST results, alignments, and sequences.
Moreover, analysis of an alignment that included plant and stramenopile sequences indicated that new and more restrictive consensuses (GDNX7RX5P and EGDX8NX5(S/P)) could be defined for Imp1 and Imp2, respectively.
"Expression-decreasing mutations yielding rate-limiting abundance changes in proteins that require chaperoning for maturation or remodeling" seems a much more restrictive (read: improbable) set than "destabilizing mutations in coding sequences".
For example, the gp38 adhesins are also quite plastic, but the sequence constraints on their modular shuffling appear to be more restrictive than those imposed on the fibrous genes.
As noted above, the physics-based model sampled with truncation selection for folding and binding is substantially more restrictive in how many non-synonymous substitutions it allows, and the simulated sequences reflect this.
A more restrictive analysis was performed to identify the effects of splicing variations on the coding sequence of alternatively spliced genes based solely on FL-assemblies for both rice and Arabidopsis.
The more restrictive SEQ subset (with 188 entries) removes this bias allowing only one representative for each group of sequences with up to 50% sequence identity belonging to the same Pfam domain.
"It's becoming more and more restrictive.
The House bill is even more restrictive.
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