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A single colonization event to the Canary Islands was supported by both the nrDNA and cpDNA trees, in agreement with previous studies based on ITS alone and/or a more restricted sampling [ 21, 22].
In contrast to earlier studies with more restricted sampling, we are unable to discriminate phylogenetic relationships between KLF/SP architectural classes composed of explicit c-LCR compositional types linked to the KLF-DBD.
However, the apparent maximum biomass concentration in the experiment with extensive repeated sampling and high volume reduction (ferm. #18a-c) was found to be slightly lower than in comparable cultivations with lower volume reduction due to more restricted sampling (ferm. #17a-c).
We, in contrast, found no close relationships between any Malagasy lineage of stick insects and those of the Mascarene archipelago and, based on a more restricted sampling, already demonstrated the Mascarene Tropidoderinae, Monandroptera and Rhaphiderus, are deeply nested within the Australasian Lanceocercata [ 20, 24].
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If this is the case, predictive power may only be found by considering more phylogenetically restricted samples.
For example, it may be that MLST data inflates r/ m estimates because it is based on far more restricted genome sampling than whole-genome approaches and so is likely to lead to lower estimates of m relative to r.
Accordingly, the output of the current study has substantial biological implications and further in-depth analysis with more temporally and spatially restricted samples will allow deeper exploration of the Cyanothece ATCC51142 proteome and almost certainly result in the discovery of additional cellular features.
We also considered HCW separately from lay participants; however, as the sampling methods for this group were different from the main community sample (the geographical area was more restricted in the HCW sample), comparisons between this group and the adult community sample should be made with caution.
Discrepancies between these and our current estimate are probably due to differences in sample size: the subtype B dataset analyzed here is geographically more restricted and includes fewer sampling points.
With these restricted samples of more proximal or representative monitors, point estimates were not meaningfully altered, and all CIs widened because of loss of subjects.
Also, changes that might first appear to characterize a large clade might turn out to be more restricted once additional taxa are sampled.
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