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We hypothesized that choosing optimal probes based on correlation with RNA-Seq TPM calls, which have previously been shown to accurately track absolute gene expression levels [ 2, 3], should lead to more reproducible microarray results than any strategy based solely on array intensities.
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Target genes that are revealed by the genomics/transcriptomics screens are generally validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR), which is more quantitative and more reproducible than microarray, requires less mRNA and is more sensitive for genes that are expressed at low levels.
The results of ADGE microarray were less variable and more reproducible than those of regular microarray.
They concluded that matching probe-sets within the same exons and using similar experimental protocols can lead to more reproducible results when performed on major commercial microarray platforms.
The nCounter results are modestly more reproducible with a median coefficient of variation of 17% compared to 25% for the microarray results, considering the same set of genes in each case.
Microarray analysis and human protein-protein interaction (PPI) network were combined to identify more reproducible sub-network markers than individual markers in breast cancer and achieved higher accuracy in interpretation of molecular mechanisms and classification of metastatic versus non-metastatic tumors [20].
In general, there was good agreement between the microarray and RT-PCR datasets for genes examined, although fold-changes were generally higher and data more reproducible for the RT-PCR analyses (Fig. 5).
New technology made Victorian sculpture more reproducible and reducible.
Google isn't alone in its pursuits to help make research more reproducible outside the lab.
The results obtained by the former method are more reproducible.
The latter are more reproducible (7.5 bp) but nucleosomes in vitro reconstitute into more fuzzy positions.
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