Anti-CD34 antibody seems to be more reliable in terms of specificity and reproducibility than monoclonal antibodies generated against other endothelial cell antigens (Tanigawa et al, 1996).
The resulting Latent Class Bivariate Model (LCBM) allows obtaining more reliable estimates of sensitivity and specificity, as well as the estimation of a different between-study correlation between sensitivity and specificity per latent class.
A more reliable way to estimate the specificity of the predictors is to predict a set of CTL epitopes and subsequently verify CD8+ T cell responses against these epitopes experimentally.
For this reason, reports of sensitivity and specificity are more reliable than tests of PPV and accuracy, which are greatly influenced by regional variation of disease prevalence.
Poor concordance between protein and transcript expression for cell-surface proteins are consistent with previous studies and, together with our data, suggest that protein is a more reliable predictor of cell-type specificity than RNA expression alone.
Our data (not shown) and others' (Robinson & Speed, 2007) confirm that the human exon array offers improved sensitivity and specificity and more reliable detection of biological variability compared with previous-generation arrays.
Since pooling sensitivity and specificity is more reliable in the absence of a threshold effect the NRA and the Genotype® MTBDR assays should be primarily judged based on their areas under sROC, while the other tests can be reliably judged based on their pooled values.
Biomarkers are becoming more and more reliable in terms of sensitivity and specificity, and even high-sensitivity assays with extended range are becoming available.
Compared to cDNA microarrays, oligoarrays provide a flexible design and are considered more reliable in terms of sensitivity and specificity [ 26- 29].
PCR screening, however, is much more reliable, faster, and efficient with higher specificity owing to effective avoidance of false positive clones identified by repeat sequences in probes by hybridization.
