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Only interactions with a combined score value ≥ 770 were considered in this analysis since they are verified as more reliable dataset containing less false negatives and false positives [28].
On the other hand, chemical analyses of the liquid and solid phases in contact (Ohnishi et al. 2017) present a much more reliable dataset because only an assumption of the local equilibrium is necessary without the information on temperature (it is noted that chemical equilibrium is assumed implicitly when melting temperatures are measured directly).
To dispose of a larger and therefore more reliable dataset, we then expanded our analysis to species pertaining to a single genus, Danio.
Our research is also based on a much larger and more reliable dataset than most previous studies.
In consequence, combining Genevestigator results with EST abundance data gives a more reliable dataset of genes specifically expressed in a certain organ, seeds in our case.
To generate a more reliable dataset of significant expression changes, we supplemented these gene lists with a further one, found by literature search [ 32].
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We propose that the use of global genomic cross-validation derived from high content technologies (microarrays or deep sequencing) can be used to generate more reliable datasets.
We propose that the use of global genomic miRNA cross-validation derived from high throughput technologies can be used to generate more reliable datasets inferring more robust networks of co-regulated predicted miRNA target genes.
However, it is still important to point out that further annotation comparisons using TIGR datasets and other primary annotations could be performed in order to establish more reliable datasets.
Performance comparison between microarray and next generation based digital expression profiling suggests that the two methodologies combined may survey the transcriptome in a better way than each on its own, and therefore generate more reliable datasets and uncovering additional new functions.
In this study 454 pyrosequencing was adopted to gain a longer and more reliable transcriptomic dataset.
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