Sentence examples for more reliable amplification from inspiring English sources

Given the large differences in the background intensity (S2 = 2 200), percent change was considered to be a more reliable amplification indicator than Δ R n.

Considering such functionally grouped reactions helps reducing the number of and selecting multiple solutions existing for each optimal objective value, enabling to identify more reliable gene amplification targets when combined with FSEOF.

Surprisingly, they found that PCR amplification was more reliable than linear amplification for detecting true expression differences between picogram input samples with higher correlation between technical replicates than linear amplification.

The INS amplicon was more refractory to reliable amplification with Taq DNA polymerase.

Our study is distinct in that we show that IVT based amplification (Ribo Amp HS) is more reliable than PCR based amplification at the sub-nanogram input level.

Increased copy number (over-representation or amplification) is technically more reliable to detect with fewer false positives than genomic loss, mainly when using FISH assay in sectioned specimens exhibiting nuclear truncation, thus we have focused on loci that were involved in genomic gain.

To make the direct readout of loop-mediated isothermal amplification (LAMP) simpler and more reliable and to enhance the visual sensitivy of LAMP detection, we developed a facile cascade signal-on colorimetric DNAzyme LAMP (dLAMP) sensor that integrates the amplification power of LAMP and the inherent catalytic activity of the DNAzyme for the simple and ultrasensitive analysis of targets.

However, based on extrapolation from the breast cancer literature, FISH is felt to be a more reliable and reproducible method for true HER2 amplification [ 18].

Although quite labor intensive, this approach allows the isolation of sufficient RNA to apply the more reliable approach including only one round of amplification, since the minimally advised starting amount is 100 ng (Ambion).

We chose to analyze the VDJ region instead of the Sμ region as the use of V-specific primers precludes PCR amplification of contaminating DNA and hence, likely yields more reliable results.

A more reliable, experimentally based approach involves the use of gene-specific 5′ rapid amplification of cDNA ends (RACE) to validate miRNA-target pairs [ 23].