In addition, we thought that many e-health opportunities for health promotion were more relevant to primary care.
Because primary care clinicians are unlikely to have the information necessary to determine whether the cognitive behavioral therapy to which they refer patients is bona fide, our results may be more relevant to primary care providers.
Indeed, the Gab/Grb2 interaction might be more relevant in primary tissues or immortalized cell lines such as BMMCs and MCF-10A, than in particular tumour cell lines often used in signalling studies, e.g. Jurkat or MCF-7 cells, which display elevated PIP3 levels due to the loss of PTEN expression or PIK3CA mutations, respectively [ 65- 68].
Although the same evaluation as the primary efficacy endpoint (i.e., change in crestal bone level from baseline) was measured at 12 months, in retrospect, the change at 12 months should perhaps have been taken as a more clinically relevant primary efficacy endpoint.
Another notable strength of the current protocol is a more clinically relevant primary end point as compared with our previous study.
This was demonstrated in the myogenic cell line C2C12, as well as in the more physiologically relevant primary culture of muscle precursor cells, termed "pp6" cells (Fig. 4).
From a clinical point of view, metastases are more relevant than the primary tumors, because metastases are responsible for the terminal illness, while primary tumors can be surgically removed in most cases.
This prompted us to explore the effect of expressing PAX3-FKHR in more relevant cells, specifically primary human skeletal muscle cells because these cells can be converted to a tumorigenic state that mimics rhabdomyosarcoma.
25 46 47 48 In addition to containing several more recent studies, our analyses have some key methodological differences that make them more relevant for the primary care population.
MSD is detected by the PHQ questionnaire which is more relevant for the primary care setting than are DSM IV criteria.
