A proteomic analysis was applied to identify the more relevant proteins expression, before measurement by ELISA.
Eight different genes involved in the NFκB signaling route were selected for methylation analysis (Supplementary Material, Table S2) according to the following criteria: on the one hand, they presented CpG-enriched promoters or first exons, which have been shown to be even more relevant to expression than the former (48).
On the other hand, women are more adept at detecting more socially relevant expressions that communicate happiness, sadness, surprise and disgust.
I understand that the authors are trying to convince readers that the differential co-expression is biologically more relevant than differential expression.
We then performed experiments in a cell line that is more relevant to the expression pattern of PNKD.
We conclude that the detection limit of this assay is dependent on the EpCAM expression, since comparable MUC-1 RNA levels were detected in all three cell lines by a faint band in the samples containing 10 spiked cells and more relevant, similar protein expression levels were observed in all three cell lines.
Therefore, in the respiratory and intestinal tract, individual CFTR expression seems to be more relevant than tissue specific expression.
Kroemer and Zitvogel previously reported that membrane expression of calreticulin was more relevant than global intracellular expression when immunogenic tumor cell death occurred in the murine model.
Apart from being expressed in tumor cells, it is also expressed in tumor-infiltrating lymphocytes (TILs), in which PD-L1 expression may be more relevant to immune checkpoint blockade response than its expression in tumor cells, i.e. a PD-1 signaling inhibitor may have no effect on a PD-L1 expressing tumor lacking an appropriate immune infiltrate.
