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Conversely, the genome-wide level of selective pressure is compatible with more relaxed selection in C. impatiens.
Low ω values were observed in structural context, indicating conservation of residues buried inside the DUF26 domain, while variable residues (under more relaxed selection) appear on the surface of the structure (Fig. 5a).
Taken together, it appears that the LTSP genes are under stronger purifying selection while STSP genes and FBX pseudogenes are under more relaxed selection.
The more relaxed selection or neutral change of this diverse collection of seeds could randomly generate new SCF E3s with different expression patterns, altered binding affinities for existing targets, or with new target specificities.
Using the Ka/Ks estimation method of Goldman and Yang [34], we found that the LTSP group of FBX loci is enriched for genes undergoing stronger purifying selection whereas the STSP and pseudogene groups are enriched for loci experiencing more relaxed selection or neutral change.
However, it is under more relaxed selection than in several other strains of S. cerevisiae.
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In more detail, selection likely acts differently, the coding regions being more likely under purifying selection, whereas the IGS may experience more relaxed selective pressure [ 4].
The higher D of heart enhancers may be due to a more relaxed purifying selection, or to a more frequent and stronger positive selection, that occurred during evolution.
Another subset was much more lineage specific, showed more relaxed purifying selection, and was enriched in loci with little or no evidence of expression, suggesting that they either control more limited, species-specific processes or arose from genomic drift and thus may provide reservoirs for evolutionary innovation.
Like FBX pseudogenes, members of the STSP group have experienced more relaxed purifying selection pressures and have weaker and more correlated expression patterns, with the group mostly devoid of FBX loci with demonstrated functional importance, implying that many are still transcriptionally active but in the process of pseudogenization.
Studies done previously with WNV demonstrate that significant intrahost genetic diversity is generated with both sequential and alternate in vivo passage; and the source of this genetic diversity is host-specific due to more relaxed purifying selection in mosquitoes [9], [10].
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