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The exceptions are the forward "generic" primer, which was re-designed to generate smaller amplicon sizes more suitable for melting curve analyses, and the T. axei-specific primer, which was modified to achieve a higher amplicon melt temperature to enable larvae of this species to be more readily differentiated from those of C. curticei.
For this experiment we used nCD4+ NKT cells, since these could be much more readily differentiated into NKT1 or NKT2 type cells (Figures 2 and 3A).
This suggests that this and similar markers will be useful in a real-time PCR methodology where positive signals can be more readily differentiated from background signals [ 15].
Similar(57)
Antigen was present in relatively few tumour cells and the amounts detected were small; it was more readily detectable in moderately differentiated than in poorly differentiated malignant cells.
For example, OPN from ras-transformed mouse fibroblasts has less phosphorylation than OPN from differentiated mouse osteoblasts and can bind more readily to MDA435, a breast cancer cell line [19].
Taken together, these data show that terminally differentiated CD8+ T cell responses are more readily detectable in HIV-1 Controllers than in HIV-1 Progressors, demonstrating a qualitative difference in CD8+ T cell differentiation between controlled and progressive chronic HIV-1 infection.
In contrast, the characteristics of L-type BSE appear to be more subtle and the results here suggest that when the signal is too intense it is not readily differentiated by visual examination from a classical BSE profile.
"I should call people back more readily.
Day care is more readily available.
Vegetable dishes ($3 to $4) are more readily identifiable.
Failures are more readily detected.
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