However, in our more quantitative validation by RT-qPCR in a larger cohort of individuals, we found that miR-151a-3p miR-151a-3p miR-151a-3pegulated in the serum of old participants.
Relating recently observed among-population heterogeneity [ 24, 26] in local infection rates to the spatial structure and environmental conditions of the entire metapopulation could consequently provide a more quantitative validation of our hypothesis.
The test cases are thought to provide a more thorough quantitative validation than previously undertaken.
Validation with more quantitative methods is an important step to guide the further improvement of these important tools.
Increasingly more quantitative experimental data permits the validation of those mathematical models, yet leads to increasingly more complex model formulations that hamper an intuitive understanding of the underlying mechanisms.
Many of the reviewed studies used large-scale microarray profiling, but follow-up and validation with more quantitative approaches often lags behind.
In general, real-time RT-PCR is more quantitative, accurate and often microarray data need validation by RT-PCR.
To estimate PM10 concentration more precisely, we adopted "leave-one-out" cross-validations to provide a more quantitative comparison of the interpolation methods (Additional file 1: Table S3).
To test the validity of the interpolation methods and provide a more quantitative comparison of the two models, we conducted "leave-one-out cross-validation" (LOOCV).
However, at the limit of somatic mutation detection with real time PCR technologies, sequencing by Sanger is not suitable as a confirmatory method and validation of results may require a more quantitative sequence variation assay.
