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More quantitative experiments are needed to address this issue.
Even if more quantitative experiments are needed to reach conclusive results, our data suggests that targeting to the plasma membrane and/or activation of a degradation pathway(s) could be very important mechanisms to regulate the activity of PtSIT2.
With the progress in our understanding of neuronal systems and the development of new and more quantitative experiments it is becoming possible to ask not only whether variability in electrophysiological properties of neurons exists, but also how large it is and what it means for neuronal function.
In the initial study with AP5 (Collingridge et al., 1983b), STP seemed less sensitive than LTP to antagonism (Fig. 1A), an effect that was substantiated when more quantitative experiments were subsequently performed (Malenka, 1991; Volianskis et al., 2013a) (Fig. 3D).
More quantitative experiments established that U2A′ did appear to increase the apparent binding affinity of U2B″ for SLIV, supporting the existing hypothesis that U2A′ function was to increase the affinity of U2B″ for SLIV.
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In order to more rigorously compare any differences in Slit binding due to deletion of Ig2, more quantitative biochemical/biophysical experiments would be needed.
However, RT-PCR results give an overall view and can be the basis to choose tissues of interest for more extended, quantitative experiments.
In an effort to examine the surface accumulation of the mutant Notch receptors in a more quantitative manner, biotinylation experiments were performed.
However, I believe that a more quantitative analysis of the competition experiment may be required to determine the degree of correlation between DN composition and virus replication fitness.
Our RT-PCR experiments provide a more quantitative look at claudin gene expression in several normal and neoplastic tissues.
In order to gain more quantitative data, we performed subcellular fractionation experiments and found that SAP30 was considerably more soluble than SAP30L, which accumulated in the nuclear matrix/chromatin fractions.
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