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MSNet identified 16% more proteins at 5% FDR than the original MS experiment (Table 2).
MSpresso identifies more proteins at the same error rate than the primary identification.
MSNet showed comparable performance across pipelines, with 10 12% higher AUC, and 7 12% more proteins at 5% FDR than the original analysis.
Indeed, when applied to yeast grown in minimal medium (Table 1, YMD-LCQ), MSNet identified 9% more proteins at 5% FDR (Table 2).
Similarly, in a precision recall plot (Fig. 2C) MSNet identified more true instances over a range of FDRs (1 precision), e.g. identifying 12% more proteins at 5% FDRref (Supplement Table S3).
This has allowed more proteins at the extremes of pI and molecular mass, as well as membrane proteins to be identified, all of which may be difficult for 2D-PAGE [ 122].
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I'm going to try to eat more protein at meals, to try to be fuller, longer.
Since protein has a greater TEF than fat and carbohydrates, those eating more protein at a meal burn extra calories after eating it.
However, the DrAqp3b-H53A/G54H/T85A constresultedulted in sligthly more protein at the plasma membrane in comparison with the other constructs when 1 ng cRNA was injected into the oocytes.
Eat more protein at breakfast.
Upon serum stimulation, however, the degradation of this protein was declined sharply and made more proteins accumulated at the 1 hr time point.
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