Clinical use of such tests is more problematic and requires access to specialist laboratory facilities, as well as a large amount of fresh tumour tissue.
This is particularly important in the case of intravitreal injection, which is more problematic for patients, requires clinician expertise, and is associated with some risks.
Deep infections are more problematic and may require irrigation and debridement with the use of intra-venous antibiotics.
The monophyletic L. obliquidens and L. obscurus are more problematic and will require the description of a new genus.
Although further validation of sequence variants in known genes may still be relatively straightforward, the identification of pathogenic mutations in novel genes will be more problematic and will require pooling of multiple unrelated patients and the use of robust bioinformatics tools (Depristo et al., 2011).
However, well-controlled patients are the target population for a looser monitoring interval (every six months) as opposed to more "problematic" diabetes patients, who may require more frequent visits.
It is more problematic than onshore wind because it requires bigger and more robust turbines, and has higher levels of maintenance and much greater installation costs than onshore wind Rising costs caused problems for the London Array, which was at the time the biggest offshore wind farm to be built.
Thus, the presence of polyploidy may be more problematic during the accumulation of cells required for a large body size, whereas other aspects of genomic stability are more critical to lifespan.
While digital systems may be readily introduced into a symptomatic role, their use in screening is more problematic because of the high throughput required for both acquisition and display.
Validating the GOF test in this setting is more problematic than for isotopologues, because it requires the identification of the fragment and because of the risk that the fragment investigated may, in fact, be derived from the coeluting compound.
