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In 34 units two or more probe tones were presented at frequencies away from CF that exceeded the accuracy of the SCF determination by two standard deviations (as in Fig. 3).
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The frequencies and levels of two or more tones were selected with reference to the RF, to be used as probe tones.
However, most of their probe tones were placed at CF. A preliminary report of a recent more systematic investigation (Stakhovskaya et al., 2008) suggested that at rostral locations within the IC, forward suppression tuning at short ISIs is affected by the frequency of the probe tone, whilst at caudal locations suppression is determined by the excitatory RF.
Instead, when probe tones are further from CF, suppressed tuning curves broaden, whilst responses to lower level probe tones are more easily suppressed by low-level conditioner tones.
In each presentation, the reference and probe tones were presented in random order, separated by a gap of 500 ms between each tone.
For both probe tones, the SCF and SBF are closer to the CF than to the probe tone frequency.
Changes in tuning were also reflected in the firing rate in response to probe tones, which was maximally reduced when probe and conditioner tones were matched in frequency.
The probe tones ran from 1.6 kHz to 4.4 kHz in 200 Hz increments, meaning there were nine probe tones of a frequency intermediate to the two reference tones, and two at either far end of the range.
This relationship was maintained for all measured gaps between the conditioner and the probe tones.
Data on binaural interactions between two tones, similarly, show that lower level probe tones are suppressed by a wider range of binaural levels (Zhang et al., 2005).
To investigate the effect of prior stimulation on the response to the probe tones they were preceded by a conditioner tone.
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