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As a result, more probe molecules could be captured and the resultant SERS signal was improved.
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The Kw values obtained correlated well with the octanol water partition coefficient and the boiling point of the more hydrophobic probe molecules, but no clear trend was observed for κG.
It is also expected that at nano-scale level there would be more anchored probe molecules and thus these would increasingly interact with target molecules which would result the strong or easily detectable signal.
Importantly, as a consequence of the generation of additional coordination space around the metal sites, more CO probe molecules could be adsorbed at the mCUSs, with higher calculated binding energies relative to the native CUSs.
Pyridine is more reliable probe molecule than ammonia, since the IR absorption bands do not overlap.
In fact, the binding protocol we use allows for the majority of the mucin (and therefore glycan) chains to freely extend into the solution, so that they are more accessible to the probe molecules (lectins) on the AFM tip (Fig. 3).
The enzymatic nature of CaNAR, wherein calcineurin dephosphorylates many probe molecules, also makes it more sensitive for detecting weak calcineurin signals.
To more rigorously control for possible interactions of our probe molecules with intranuclear structures, we performed FLIP experiments.
Nevertheless, the successful synthesis of potent biologically active probe molecules will serve as a starting point for initiating more sensitive methods of probe-based target identification.
In order to get more insight into the characterization of nanoporous carbons by gas adsorption, the use of different probe molecules has been compared.
Adsorption of the probe molecules cyclohexane and n-heptane indicated a strong decrease in accessibility for samples with more than 3 wt% loading of phosphorus species.
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