Compared with the batch reactor, the microfluidic system provides a more precise control of reaction conditions, faster reactant mixing and enhanced mass transfer.
This more uniform and exact termination of experimental treatments likely provides more precise information regarding dynamic reactions that occur in rapid time courses, exemplified by many phosphorylation reactions.
For more precise quantification, targeted PCR reactions were carried out, and the amplified products were analyzed by automated chip-based microcapillary electrophoresis on an Agilent 2100 Bioanalyzer instrument.
For a more precise determination of active reactions in each growth condition, we performed flux variability analysis (FVA) [ 44] to determine possible ranges of fluxes of all reactions that still satisfy the same optimal growth.
For more precise quantification, targeted PCR reactions were carried out, and the amplified products were analyzed by automated chip-based microcapillary electrophoresis on an Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA) as previously described [ 37].
This novel approach allows us to reach a more precise control in the biological reaction, with a beneficial effect on process efficiency, actually introducing a new class of devices.
In fact, more precise quantification by targeted PCR reactions and analysed of the amplified products by chip-based microcapillary electrophoresis indicated that the ratio of DR3Δ6 to full length DR3 doubled in SW620 cells relative to SW480.
While the dials seem like a natural extension of focus groups, if not a more precise gauge of real-time audience reaction, several producers dismissed them as the most simplistic and desperate research tool yet, the enemy of groundbreaking work.
To be more precise, we do not consider the reactions occurring in vitro (namely, entries 28 and 35 in Table 1 in [ 30]), as well as the reactions downstream the fibrinogen conversion, that is, the interactions between the fibrin polymers, thrombin, and antithrombin III (namely, entries 55, 56, and 57 in Table 1 in [ 30]).
More precise data, number of aggressive / positive reactions per test, in overall were also compared but proved mostly non significant (p>0.05 in all cases) and results will therefore not be described in details here.
