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The average removal efficiency from the tests was greater than 99% with more precise quantitation limited by the sensitivity of the assay.
These results are readily visualised as presented, and we believe the investigation does not need more precise quantitation as suggested by the reviewer.
The other HPLC/MS method capable of determining eight sweeteners including acesulfame-K, alitame, aspartame, cyclamate, neotame, saccharin, sucralose and stevioside allows for more precise quantitation of analytes warfarin sodium has been applied as internal standard [ 23].
The dynamic range of expression obtained by messenger RNA sequencing can be much broader than that obtained by gene expression microarrays, allowing the detection of rare transcripts and more precise quantitation of expressed transcripts.
With AID, APOBEC3C and APOBEC3G the yeast data essentially confirm the previously identified sensitivity to nucleotides located at positions −1 and −2 (AID: 5′- WR C, APOBEC3C: 5′- TY C, APOBEC3G: 5′- CC C) whilst allowing more precise quantitation of the degree of preference.
These methods, the production of additional data sets for comparison, and the incorporation of control background data [ 87] may allow for more precise quantitation of binding activity from CLIP data, which could reflect the stability or half-life of RBP-RNA interactions, and provide quantitative data to aid motif finding.
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The use of several levels of separation and the generation of a relatively high number of peptide fractions led to the identification of a significant number of proteins and of low-abundance proteins, as well as to more precise sample quantitation, as the coisolation issue related to iTRAQ tagged peptides is less pronounced in fractions with reduced complexity [ 24].
Thus, counting only the cells depleted of LTAg should result in much more accurate quantitation.
A more accurate quantitation of MPL expression was undertaken using qRT-PCR.
In a few situations, outliers were removed for more accurate quantitation.
The one-step real-time PCR based on the LightCycler technology is more rapid and less contamination-prone than the nested RT-PCR and allows the precise quantitation of the virus load in the tested specimens.
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