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Compared to culture-based techniques, DNA sequencing of the bacterial 16S ribosomal RNA (rRNA) gene or whole metagenome shotgun (WMS) sequencing provides more precise microbial community characterizations.
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Microbial genetics is enabling a more precise definition of bacterial, viral and parasitic threats - and how they develop resistance to treatments.
Although very useful for taxonomic, physiological and genetic studies, culture-based techniques are insufficient for a more precise characterization of the functional and phylogenetic diversity of microbial communities, since it is now well known that only a small fraction (0.1 to 10%) of the microbial diversity in nature can be recovered in the laboratory (Torsvik et al. 1990; Amann et al. 1995).
The newest developments of metabolomics, meta-omics, computer, and mathematic sciences offer more options for not only unbiased selection and ranking methods but also high-throughput and more precise prediction models that enable a mechanistic description of microbial metabolic pathways (Breitmaier 2006; Martin et al., 2003).
These newly developed in situ measurement tools will enable more precise estimates of the energy states of habitats of chemolithotrophic microbial communities.
The authors put emphasis on microbial keratinase because it is less expensive and more precise than those conventionally used chemicals to treat keratin.
Factors such as increased microbial virulence, increased day-care attendance, improved microbiological diagnosis and more precise ICD-coding might all be important factors to explain these historical differences.
Community-level studies have become more precise with the application of culture-independent methods based on the direct detection of DNA in microbial ecosystems.
Previous studies have included direct assessments of oral (39) or gut (40) microbial exposures to study either cardiovascular or obesity risk; similar approaches can provide more precise characterizations of infection insulin resistance associations in future studies.
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