However, to obtain a reliable placement of the nodaviral RdRp, we will probably have to wait for more powerful sequence-based phylogeny approaches [ 78], or for the resolution of its 3D structure, to which novel structure-based phylogenetic approaches could be applied (e.g. [ 79, 80]).
The discrepancies in the aforementioned studies highlight the need for more rigorous and more powerful sequencing approaches coupled to the identification of antigen-experienced T cell subpools.
Recently, the new generation machine, GS-FLX, possesses more powerful sequencing capability of up to 200-300 bperereadandand higher sequencing accuracy than that of GS20 http://www.454.com.
To assist in closing the gap in our understanding of this complex pathogen, the advent of more powerful sequencing techniques and the ability to knockout parasite genes allow more systematic assessment of the parasite genome and already have revealed that there are genes for many parasite proteins that have not been recognized previously.
Since first introduced by Pellegrini et al. [ 4], Phylogenetic profiling has been successfully applied to the prediction of protein function by several groups and demonstrated to be more powerful than sequence similarity alone at predicting protein function [ 5- 11].
As the depth of sequencing improves with the advent of still more powerful HT sequencing technologies, we envision that this feature might be integrated in future miRNA discovery pipelines.
Second, in general, the measure seems to become more powerful for sequences with increased GC content, because the higher the GC, the less likely it is that a reshuffled sequence shows reduced stability.
These important gaps are likely to be bridged over the next several decades using (1) much larger sample sizes, (2) more powerful RNA sequencing and proteomic methods, and (3) novel statistical and computational models to predict genome-to-phenome relations.
Consequently, it has been superseded by more powerful spectral sequences with fewer non-zero differentials, which give more information.
In addition, our method is easily updated and made more powerful as newly sequenced chloroplast genomes are added to the reference database.
