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HPEC show a spindle-shape that largely differs from the more polygonal phenotype of HUVEC [ 33– 33].
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Phase-contrast microscopy analysis of ASCs cultured with 5 μM all- trans retinoic acid (ATRA) for 11 days showed a gradual conversion into a more epithelial polygonal phenotype with respect to untreated controls.
The cell micro morphologies on the ZnHA/TiO2 coatings were more polygonal compared with that on HA/TiO2 coatings (round) by SEM.
Additionally, vorinostat-treated cells featured a more polygonal shape and less apparent invadopodia than control cells.
EBF between passages 16 – 20 started to change their morphology: cells were large, flat and more polygonal shaped, with a large, heterogeneous nucleus.
Accordingly, p130Cas silenced cells reorganized in colonies that lost their elongated protrusions, acquiring a more polygonal shape, as quantified by a marked decreased in length/width ratio.
Tubules formed at 21% O2 by both cell populations were more polygonal in shape, less likely to be fully enclosed, and typically bordered by a layer of only one cell (Fig. 4c, f).
After three weeks culture time with the osteogenic induction medium the cells changed to a more polygonal appearance, formed nodules and were stained positive with von Kossa stain for mineral deposition (C).
They are arranged in bands (putative intercostal regions) and are surrounded by more polygonal-isodiametric cells.
Particularly MG-63 cells on the hydrophilic, nanofibrous 16% + F68 scaffolds developed a more elongated phenotype.
Antisense suppression of OsPIP1 1 causes partially defective phenotypes during seed germination, but overexpression of that gene does not stimulate the occurrence of a more normal phenotype.
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