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However, GS appears to be located in a more polar environment nearer the bilayer surface when cholesterol is present.
Chls with phytyl chain were located in the micellar core, where the micropolarity is similar to ethanol, while phorbides' derivatives (without phytyl chain) were located in the outer shell of the micelle, i.e., more polar environment.
Furthermore, we observed a pressure-induced displacement of the emission maxima of a solvatochromic fluorophore (7-diethylamino-3((((2-maleimidyl)ethyl)amino carbonyl) coumarin) placed at the same positions, which suggests a relocation to a more polar environment.
The absence of a green shoulder in the emission spectra of nucleoli is in accord with the more polar environment in ribonucleoprotein assemblies (Fig. S3 A in Data S1).
Additionally, NaAn (I) has a NH2 group attached to 2- position that would prefer to remain in more polar environment due to its polar nature and the geometric hindrance.
The highest emission maximum was observed for H956A signifying that the tryptophan residues in this particular protein conformation exist in a more polar environment relative to C997A and WT NS2-3.
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OxP-11D had a less defined conformation in more polar environments, however in hydrophobic solvent systems and in lipid multilayer ensembles, including these with cholesterol, the peptide showed highly ordered helix and turn structures.
Overall, type I reactions become more important at low oxygen concentrations like inside of biofilms or in more polar environments [ 46].
During this transition, neuronal UCPs exhibited small overall conformational changes and their Trp residues were exposed to more polar environments (λmax ∼ 338 339 nm).
Examination of the λmax of Trp compared with [SDS] revealed that Trp residues experienced a red shift towards higher wavelengths (335 339 nm) at SDS concentrations between ∼5 8 mM, signifying the exposure of Trp residues to more polar environments.
This "low dielectric" environment is likely responsible for the elevated redox potential of Trp382 relative to Trp359 and Trp306 (see Figure 13B), which are in more polar local environments that include H-bonding to water.
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