Sentence examples for more physiological system from inspiring English sources

To confirm these results in a more physiological system, cell lines stably over-expressing a low level of myc-tagged raptor alleles with stable knockdown of endogenous raptor with short hairpin RNA that targets the 3' UTR of raptor [16] were generated in Hela cells.

In our experiments, we chose to use Transwell co-culture experiments to resemble a more physiological system and also to test functional miRNA transfer with reporter constructs.

As these studies were based on overexpression systems and are conducted in yeast or non-T cell lines, extension of these findings to a more physiological system would help improve our understanding of this important event.

However, recent reports suggest the TGA is superior for assessing coagulation status because it involves more physiological systems and measures total thrombin generation over time [ 5– 7].

When approaching more physiological systems, the combination of a heterogeneous, multi-component structure (and non-affine deformations), complex feedback mechanisms down to the molecular scale (e.g. force-dependent cross-links), and out-of equilibrium properties (e.g. motors or polymerization) drastically complicates a profound understanding of network mechanics and organization.

We next conducted surface plasmon resonance analyses, a more physiological assay system, to further define the CFH domain interacting with HcpA.

In our study we used a more physiological model system using primary human cells from typed individuals without the need for genetic manipulation.

Finally, for selected compounds we used colony growth assays with normal human bone marrow to verify tumor-specificity in a more physiological model system.

Thus modelling of tumour biology is cell-type and stroma-context dependent, and alterations of these from a 2D to a more physiological 3D system produces striking changes.

I also had the opportunity to try advanced imaging methods such as total internal reflection (TIRF) microscopy and the use of more physiological cell systems.

To advance our understanding of the impact of these gyrA/ parC mutations on S. Typhi epidemiology, we suggest validating our findings in more illustrative physiological systems, such as the S. Typhimurium biliary carriage model (Crawford et al., 2010) or a human S. Typhi challenge model (Pollard et al., 2012).