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To extend our findings into a more physiological setting, we generated mouse tumor xenografts using our set of mESC lines, and treated the mice with topotecan on a previously established schedule29.
Those models may facilitate efforts to improve therapeutic outcomes for malignant melanoma by providing a platform for the investigation of cytotoxic treatments and tailored therapies in a more physiological setting.
Since PKCθ−/− aggregated normally, despite increased αIIbβ3 activation and α-granule secretion, we investigated whether the role of PKCθ might become more apparent during thrombus formation in the more physiological setting of flow conditions.
To verify whether VPAC1 protein expression was indeed regulated by miR-525-5p miR-525-5p miR-525-5pal setting, U937 cell line, in which miR525-5p was not expressed but into which hsa-more525-5physiologicalntly transetting, were harvested at different times and analysed for VPAC1.
This indicates that the absence of the CD-loop is also redundant in the more physiological setting of the cell surface.
The thermodynamics of these processes remain poorly elucidated and converting the in vitro findings to a more physiological setting is required.
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In the more physiological set up of an infection, the collaboration between TLR and BCR signaling might be important for the early activation of pathogen-specific B cells that help contain the infection until the establishment of a mature T cell response.
This is an unphysiological situation; nor were the constructions tested with cyclical loads, which would have been a more physiological set-up.
We found two more relevant and more physiological data sets, i.e. ex vivo virus infection-induced profiles in PBMC from infected children.
Since branched actin at the cell cortex integrated the three inputs, growth factors, cell-substratum adhesion and cell density, we then tried to induce differentiation of the potentiated cells with more cortical branched actin in a physiological setting.
A third possibility is that the transcription factors in the embryo data sets have many more physiological target genes in common with each other than with Ubx and Hth.
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