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This technique builds on previous capsular bag models, providing a more physiological model for observation of PCO in vitro.
Acute resistance exercise is a more physiological model of muscle loading than synergist ablation.
We also sought to explore a more physiological model of intracellular invasion than direct microinjection.
In our study we used a more physiological model system using primary human cells from typed individuals without the need for genetic manipulation.
Conceptually similar observations were made in a more physiological model of oncogene-induced senescence in which an inducible endogenous K-RasV12 allele drives the development of adenomas (pre-malignant) and adenocarcinomas (malignant), being characterized the adenomas by a strong senescence response [37].
The data presented here suggest that different miR-200 family members do not inhibit migration but have distinct effects on the mode of tumour cell migration when studied by invasion into a 3D collagen-I matrix, a more physiological model of the interstitial matrix.
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Here we discuss how the two approaches can be combined to generate more physiological models of stem cell niches that could facilitate the identification of new mechanisms of stem cell regulation, profoundly impacting drug discovery and ultimately therapeutic applications of stem cells.
Further experimentation in more physiological models and in human heart cells will examine the efficacy of these ideas.
The relevance of these results needs to be verified in more physiological models, especially since NLRC4 activation was achieved by a highly dosed and artificial ligand.
(ii) Present/absent detection calls for gene expression from more physiological models can be used to constrain the list of possible molecules for network construction.
We named this configuration E. Secondly, we developed a more physiological network model including also inhibitory connections; we named this configuration EI.
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