Transforming growth factor-beta1 (TGFβ1) induces myofibroblastic activation in mitral valve interstitial cells (MVIC) in static 2D culture, but the roles of more physiological 3D matrix and cyclic mechanical strain are unclear.
The data presented here suggest that different miR-200 family members do not inhibit migration but have distinct effects on the mode of tumour cell migration when studied by invasion into a 3D collagen-I matrix, a more physiological model of the interstitial matrix.
In the more physiological 3-D system, the cells exhibit matrix adhesions all over their surface, and 3-D cell-matrix interactions enhance cell biological activity [40].
The novel barrier cell migration assay offers the possibility to analyze cell migration on defined ECM proteins, and thus represents a simple device to investigate the role of cell-matrix interactions in migration of adherent cells under more physiological conditions.
Furthermore, we seed different types of MyoFbs into 3-D collagen matrices (3-DCMs) to identify the role of mechanical stress and growth factors in a more physiological environment and to further study the potential for dedifferentiation of MyoFb.
We thus aimed our study at investigating the effect of a low (more physiological) oxygen percentage both on the cartilage tissue forming capacity of human articular chondrocytes (HAC), and on their pro-catabolic, matrix degradative activity.
It's more physiological than that".
ETV is a more physiological treatment than VPS.
Thus, simultaneous quantification of bioactive ingredients in a complex physiological matrix is crucial.
There is an on-going debate which type of loading is more physiological.
