Sentence examples for more physiological assay from inspiring English sources

We next conducted surface plasmon resonance analyses, a more physiological assay system, to further define the CFH domain interacting with HcpA.

While promoter activity could be detected for a number of the constructs, the absence of evidence of cell-type specificity, i.e. absence of clear and consistent differences in promoter activity for the various constructs between the retinal and non-retinal cell lines, led us to utilize a more physiological assay for assessing retinal promoter activity.

This method is more physiological than luciferase assay as it measures the expression of target gene from their natural genomic context, although it can not discriminate between direct and indirect effects of miRNA expression.

Having demonstrated binding of CFHRs to intact borrelial cells, the role of CFHR for complement resistance was assayed under more physiological conditions.

Further reduction in the numbers of such animal tests may be achieved if more physiological data can be obtained from assays in simpler organisms such as zebrafish.

Furthermore, to confirm whether this can also occur in more physiological conditions with serum present, the CFE assay was utilized.

To address the question of whether AMP is capable of competing with ATP at the allosteric sites, we also conducted assays not only at 200 μM ATP as in the standard assay, but also at the more physiological ATP concentrations of 1 and 5 mM.

DOI: http://dx.doi.org/10.7554/eLife.10451.005 To gain mechanistic insight into the nature of this regulation in a more physiological setting, we performed adenylyl cyclase activity assays using membrane isolated from mouse striatum.

We sought to perform the endocytic assay in unperturbed cells, under more physiological conditions, by omitting the pre-incubation step at 4°C.

Initial analysis of 4p-PTEN catalytic activity was carried out with a soluble PIP3 substrate containing hexanoyl rather than the more physiological palmitoyl chains using a phosphate release detection assay with malachite green (Van Veldhoven and Mannaert, 1987).

To examine the protein interactions under more physiological conditions, we then performed a pull-down assay using mouse brain lysate because, among various tissue types, Lamp2c mRNA showed the highest expression levels in the brain (Fig. 1D).