Sentence examples for more phosphorylated peptides from inspiring English sources
As a result, ERLIC identified significantly more phosphorylated peptides in the HeLa digest.
More importantly, most phosphorylated peptides from α-S1-casein and α-S2-casein were identified by mass spectrometry (Table 1), demonstrating the capability of VIPing-based enrichment of phosphopeptides.
On the other hand, reversing the order of the separation methods (Fe3+-IMAC followed by HILIC) results in significantly lower phosphopeptide enrichment, with more non-phosphorylated peptides being present (McNulty and Annan 2008).
For a more elaborate presentation of differentially phosphorylated peptides, see Text S1.
Multiply phosphorylated peptides were more likely to be detected by SAX than by SCX coupled with TiO2-MOAC (Nie et al. 2010).
MBP, which is the major structural component of the myelin sheets in the CNS, and alpha-crystallin B (CRYAB) were identified with differential phosphorylation by two peptides, being one more phosphorylated and the other less phosphorylated.
The relevant clue related to ZrO2 is that it permits the isolation of single phosphorylated peptides in a more selective manner than TiO2 [12–24].
This is likely caused by the significant reduction in sample complexity after enrichment of tyrosine phosphorylated peptides, thus allowing a more comprehensive and reproducible profiling of tyrosine phosphorylation [22].
Among the phosphorylation sites that were independently identified by MS/MS in all six elutriated fractions, 89 phosphorylated peptides varied in abundance by more than twofold (∼3% of phosphorylation sites detected, Supplementary file 5).
Therefore, specific enrichment of tyrosine phosphorylated peptides by phosphotyrosine-specific antibodies may provide a more targeted approach to study tyrosine phosphorylation [20] [22].
23% (489 phosphorylated peptides) of all quantified phosphorylated peptides displayed a more than 3-fold up-regulation, whereas only 3.5%7878 phosphorylated peptides) displayed decreased levels.
