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Beeman and the rest of the production staff reviewed the scene to make sure that they filmed the homage correctly, but Beeman later noted that the iconic scene was "clunkier" and more "out of focus" than they wanted, and that people's memories make it seem more impressive.
"It's more and more out of focus for me now.
Keep in mind that the more out of focus your picture, the larger the shapes.
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The loss in axial performance with the half-pupil is recovered in the divided-pupil line-scanning configuration (Δz sl ≈1.30μm) providing better sectioning as more out-of-focus light is suppressed by the confocal slit or pinhole.
As analyzed earlier for Eq. (1), the 920nm laser intensity at the focus is much more intense than the out-of-focus counterpart at the imaging-depth limit.
As analyzed earlier, I e x c (r, z, t ) at the focus is much more intense than its out-of-focus counterpart when reaching the depth limit of regular 2P imaging.
Both MPDI and MPAI of RSFPs are based on the insight that the laser intensity at the focus has to be much more intense than the out-of-focus counterpart despite the scattering loss, when the depth limit is reached for regular fluorophores as defined in Eq. (1).
It automatically weeds out duplicates, out-of-focus images and more, and also uses geo-tags and landmark detection to figure out where you are.
LSCM removes this out-of-focus haze and shows more detail (Fig. 1B).
Infants who pointed produced more co-occurrences of gaze, vocalizations and points in the Out-of-focus condition than in the In-focus condition.
Most importantly, out-of-focus blurs are: Much larger and more diffuse at wider apertures.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com