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In this work, we identified more optimal tracers, namely [2,3,4,5,6-C] for the oxPPP flux and [3,4-C]glucose for the PC flux. Figure 5A displays the confidence intervals for the oxPPP flux for Henry's tracers and our proposed tracer.
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Therefore, there is a pressing need for new rational approaches for designing tracer experiments to systematically identify optimal tracers, or at least reduce the search space to a computationally more manageable level.
To date, no optimal tracers are available for this application.
Based on this extensive analysis we have identified optimal tracers for 13C-MFA.
Optimal tracers for the analysis were selected by evaluating the immunochemical activity.
However, as of today, no systemic methods exist for identifying optimal tracers for parallel labeling experiments.
From a select list of available isotopic tracers, Metallo identified optimal tracers for specific metabolic pathways.
The feasible tracers were evaluated using numerical simulations to identify optimal tracers for elucidation of both oxPPP and PC fluxes.
In this work, we used the EMU tracer experiment design approach to select optimal tracers in the described system.
We predicted two novel optimal tracers, which were not previously considered for mammalian systems.
However, to address the tracer experiment design problem, i.e. how to select optimal tracers to accurately estimate fluxes in the model, additional information is needed.
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