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To investigate whether, despite the lack of more obvious mutant phenotypes, ceh-63 is in fact required for DVC specification and development, DVC was visualized in the ceh-63 deletion background using ceh-63::gfp fusions.
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Similar with rps19 mutants, the dissociation of RNA and protein levels of mCherry was more obvious in rpl11 mutants of mCherry control transgenic line.
After 3 days, the yellow-green phenotype was more obvious in egy1 mutants (Fig. 5d) than that in WT plants (Fig. 5c).
This is more obvious in chz1 mutants suggesting that Chz1 partly contributes to the Htz1-binding specificity.
For all the biochemical assays, we chose the "LFL" mutant as the mutation produced more obvious impacts (i.e. at similar levels to those of the site-1 or-2 mutants) in the target bindings via the site-3.
Furthermore, the yellow-green phenotype of egy1 mutants is more obvious than that of wild-type (WT) plants with increasing leaf age, suggesting an early senescence in the egy1 mutants.
Furthermore, after darkness treatment, the yellow-green phenotype of egy1 mutants was more obvious than that of WT.
The misalignment of the hair cells in the apical half of mutant cochlea was more obvious in later stages (P7 shown here) and demonstrated two rows of IHCs and four to five rows of OHCs (Fig. 5F,F').
In terms of leaf length, the effect of the slr1 mutation was much more obvious in the wild-type background than in the pla1 mutant background.
Then, cooperated with analysis of snapback primer by high resolution melting (SPACE-HRM), mutant peaks must be more obvious, while wild peaks are weakened.
This X chromosome bias is even more obvious for genes required during spermatogenesis: of over 40 mutants with spermatogenesis defects, none are X-linked (S. L'Hernault, personal communication).
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