This will enable systematic structure-function studies for genes, for which no mutants are available, or more importantly mutants that display highly pleiotropic phenotypes.
Birds are also more susceptible to MDV mutants that have emerged in regions with more-virulent strains than to previous MDV isolates.
In our experience mutations that improve fluorescent brightness are much more readily identified than mutants that improve photostability.
aSyn was shown to induce aggregation of several yeast Rab GTPase proteins and this aggregation was more pronounced in yeast mutants that produce high levels of acidic phospholipids.
Dinucleotide repeat array expansions (Morel et al. 1998) as well as "plus" frameshifts and duplications (Nagata et al. 2002) occur more frequently in polA mutants that are defective in the 5′ 3′ exonuclease activity of the enzyme.
More recent studies described 2 C3 mutants that bind CFB with higher affinity, resulting in a more stable C3 convertase complex.
Increasing the read depth to 6 million reads per condition detects 96% of mutants that change more than two-fold, 91% of all mutants that change more than 1.5-fold and 72% of all mutants that are significant in the full experiment.
Mutants that have more severely altered activity in vitro (both fast and slow) show greater growth defects, more genetic interactions, and greater alterations to gene expression profiles in vivo.
In a systematic analysis of the adhesive and invasive capabilities of 1084 mutants, 10 mutants that showed more than a 50%% reduction in adhesion or invasion were obtained.
In addition, although Serratia endonuclease is most active with Mg++, mutants that are more active in Mn++, Co++ and Zn++ have been isolated [54].
