There are other, more comprehensive cDNA microarray methods available, which produce copious amounts of data.
While this is a weakness of the currently available Citrus arrays, non-attributable to the array-CGH procedure, more complete results are expected after the development of a more representative cDNA microarray.
More recently, cDNA microarrays representing 2,366 unigenes were used for an analysis of nodule development in the M. truncatula R108 line coupled with the independent analysis of a collection of 15 plant and bacterial mutants inducing non-functional, Fix− nodules [28].
A multitude of methods have been used for the characterisation of pre-invasive breast lesions, including immunohistochemistry, fluorescent in situ hybridisation, analysis of loss of heterozygosity (LOH), comparative genomic hybridisation (CGH), and, more recently, cDNA microarrays and proteomics analysis.
Transcript profiles for 9 normal male and 10 normal female controls were measured using spotted cDNA microarrays with more than 44,000 cDNA elements representing 26,000 unique genes.
In recent years, long oligonucleotide microarrays have become more popular than cDNA arrays because the generation of cDNA microarrays involves many laborious and error-prone steps, including bacterial culturing, PCR amplification and purification, and DNA sequence validation [ 2, 3].
More importantly, a cDNA microarray study identified a gene expression signature that separated CN-AML into two prognostically relevant subgroups (Bullinger et al, 2004).
More recently, a cDNA microarray study of PBMC from 10 individuals in Mexico having urinary As levels between 117.23 and 435.12 mg/g creatinine (n = 5) revealed significant differences in expression of apoptosis- and inflammation-related genes compared to unexposed subjects (n = 5) [ 22].
cDNAs that are complementary to probes on the microarray hybridize on the basis of simple first principles: more highly expressed genes will have more transcripts converted to labeled cDNA, and these more abundant cDNAs will bind more to their target probes than those of less expressed genes.
Future expansion plans of the database include recruiting more indica cDNA clones, cataloging splice variants and adding gene expression data from indica cDNA microarray.
