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The established protocol contains a step for lipid removal and a precipitation step that renders this procedure more laborious compared to the FASP and RapidACN protocols.
Although the optimalization of such experiments is more laborious compared to reactions using dilution into water (there is higher possibility of non-specific amplifications from the volunteer DNA) we believe this approach better reflects the real situation, offers more reliable results and should be used in all backtracking studies.
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The first protocol is based on the proprietary, acid degradable detergent RapiGest (Waters ), included in a protocol derived from Von der Haar et al. This protocol involves protein precipitation, which renders the RapiGest procedure more laborious as compared to the second in solution protocol, termed RapidACN.
Furthermore, MLVA subtyping was more rapid and less laborious compared to PFGE.
These methods can be more accurate and, when automated, less laborious compared to the standard curve method [ 43].
Additionally, it is a more rapid and specific method of MTC speciation apart from being less laborious compared with biochemical, phenotypic and IS 6110-restriction fragment length polymorphism (RFLP) analysis [ 22].
Histological analysis of a biopsy sample, more laborious in preparation and study as compared with that of a cytological smear, is also not absolutely efficient owing to a substantial rate of interobserver discrepancies among expert pathologists examining the same material [ 4].
The flow reactors have been applied to the production of both inorganic nanoparticles (silver nanospheres) and organic vesicular systems (liposomes), and their performance compared with reactors produced using more laborious fabrication methods.
By providing these profiles, PhyDesign facilitates locus prioritization increasing the efficiency of sequencing for phylogenetic purposes compared to traditional studies with more laborious and low capacity screening methods, as well as increasing the accuracy of phylogenetic studies.
A more direct (although more laborious) method of calculating access by non-subscribers would be to analyse the log transaction files of the publisher and to compare the list of internet protocol addresses from subscribing institutions with the total list of internet protocol addresses.
Another option is a little more laborious.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com