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Therefore we cannot exclude the existence of more intermediate clade genes in P. abies.
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There will be 5,000 more "intermediate care" beds by 2004.
Thus, the expression of PaWOX8/9 and PaWOX8A seems to be comparable to the A. thaliana intermediate clade genes although their expression is more limited to embryo development.
The lack of amplification of DNA from most intermediate strains is not unexpected because their Leptospira species form a separate intermediate clade situated between pathogenic and saprophytic species [23], [32].
The analyses showed good support for the three major clades of WOX genes, the ancient clade, intermediate clade, and modern clade.
The other two clades are termed the intermediate clade, containing AtWOX8, 9, 11 and 12, and the modern clade, containing AtWUS and AtWOX1- 7[ 1].
In the intermediate clade there were no clear P. abies orthologs to the angiosperm sequences.
The intermediate clade consisted of four PtrWOXs; these were classified into two subclasses: PtrWOX8/9, and PtrWOX11/12.
More intermediate concentrations should be shown.
The three clades were: a) the derived clade, containing 279 sequences from 26 species; b) the intermediate clade, containing 111 sequences from 25 species; c) the ancient (basal) clade, containing 29 sequences from 20 species.
For the intermediate clade and modern clade sub-trees the analysis was run for 3.000.000 generations with a burn-in of 600 and 1250 respectively.
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