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The more intense pigmentation in floral parts of Lc-transgenic tobacco plants (Fig. 1A) should be due to systematic effects of ectopic expression of Lc.
In this study, heterologous expression maize Lc in tobacco exhibited more intense pigmentation in the floral tissues (Fig. 1A) similar to the previous report by Lloyd et al. (1992) and Yang et al. (2007).
The specific expression of Lc in the calyx, petals and filaments increased the transcript abundance of anthocyanin biosynthetic genes (Figs. 2, 3), which resulted in the higher anthocyanin content, and led to the more intense pigmentation in floral parts (Fig. 1).
The constructed mutant protein, designated BphS (SI Figure S2), produced more intense pigmentation on Congo red plates than BphS1.
This may be attributable to a more intense pigmentation conferred by the HGF+ genotype to tail skin of both the HGF+ and HGF+ [ m1m2]+/− hybrid strains.
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