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Immunohistochemical analysis of the kidneys showed secretion of renin occurring in the donor but not in the recipient twin, more intense expression of angiotensin II receptor type 1 in the donor, and modifications of renal architectures in both twins.
Tomato expression in placentas was observed in all trophoblast cell lineages (Fig. 3); however, it was not uniform across all trophoblast cell lineages, with more intense expression occurring in spongiotrophoblasts and giant cells (Fig. 3).
On tissue section, VEGF immunolabelling revealed a more intense expression by osteoblasts and osteoblast-like cells, in addition to its expected expression by hypertrophic chondrocytes.
Moreover, Garlet et al. [ 15] observed equal prevalence of OPG expression in periodontitis patients and control, even a more intense expression was detected in the diseased group.
Nestin immunoreactivity showed a more intense expression in NOBEC compared with OEC in all culture conditions and the addition of GDNF determined an upregulation of nestin both in serum and in SFM in NOBEC and OEC.
Likewise, this HSPG was significantly elevated in abundance at dfmr1 null synapses compared with in controls, exhibiting a more intense expression closely overlapping with the HRP signal (Fig. 1C).
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Evaluation of care provided to ethnically diverse patients often showed a "magnifying glass effect" [ 31]: difficulties in the care process are revealed that are not unique to patients from these groups but are more intense expressions of general paediatric care problems.
Hence, the joint association observed in this study among AFP, GGT, liver fibrosis and SVR may be the reflection of more intense HPC expression in non responders compared to responders.
Importantly, it was found that a more intense protein expression is paralleled by a higher intensity of the mRNA signal, both in kidney tissue and USC (Table 2), and also that KIM-1 mRNA expression both in tissue and USC increased from IF/TA category I to III (Table 3).
Increased GFAP expression was also seen throughout the hippocampus, where astrocytes in all hippocampal regions, especially the SLM, displayed more intense GFAP expression.
Consequently, using enhanced green fluorescence protein (EGFP) as a reporter gene, DLCS-NP-treated human conjunctival epithelial cells exhibited 3.1- and 3.5-fold more intense EGFP expression than that of LCS-NP and CS-NP, respectively.
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