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If this error rate applied to the sequencing of the entire human mitochondrial genome comprising about 16,570 base pairs, then the majority of complete mtDNA sequences in a database would carry one or more incorrect bases – which was typically attained by the earliest sequencing attempts of the past, but most recent clinical mtDNA studies do not fare better and sometimes much worse [3] [5].
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Nothing could be more incorrect".
He could not be more incorrect.
And there is something even more incorrect, even more unsayable than this.
Trainwreck hasn't got the acid of Apatow's more incorrect comedies: some laughs, though.
The PCR process often substitutes incorrect bases for ones that have been chemically derivatized.
MR rarely finds more incorrect splits than MRP.
In contrast, MRg and find up to 1.9% more incorrect splits, while MR and find up to 0.8% more incorrect splits.
Detecting the correct signal in a homopolymer of four or more bases can be problematic, resulting in incorrect base calling.
At an overall sequencing error rate of 1% and a coverage depth of C = 20, 30, 40, and 50, assuming a base has an equal chance to be mis-sequenced to one of the three other bases, the chance that an incorrect base is observed twice (three times or four times) or more is shown in Table IV and Figure 4.
However, incorrect base calls in islands, especially near island endpoints, would cause many more junctions to be missed, a problem that was greatly reduced by the use of the reference bases within our assembled islands.
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