Further investigation of this cluster to identify more homogenous sub-clusters might, therefore, be of interest.
The dendogram also reveals two more homogenous sub-groups of Cluster 2. One of the sub-groups is characterized by abnormal protein whereas patients in the second subgroup showed personality and behavioural changes.
However, it can be seen from the dendogram that there are three relatively large clusters that can be divided further into more homogenous sub-groups by choosing a slightly smaller cut-off point.
However, studying a more homogenous sub-population will require (i) even larger overall sample sizes to overcome increased multiplicity and stratification, and (ii) more detailed data on phenotype, lifestyle and environmental factors.
To examine if the gender difference in seropositivity could be explained by high-risk women being more likely to present for testing, we assessed how the HIV risk ratio between women and men varied in more homogenous sub-populations (e.g., age 20 30; not widowed; or no partners with other partners).
Future research could aim to further dissect these regions (e.g. by laser capture) so that more homogenous sub-regions could be studied.
Individual clades of the hierarchical tree were subjected to K-mean clustering to get more refined sub clusters.
We have also observed that the Ni thickness below 4 nm is preferable for more homogenous Ni cluster size distribution and consequently CNT diameter distribution.
After 21 days of culture, cell clusters were observed in all hydrogels with longer PA chains and these hydrogels supported more homogenous and established clustering as well as significantly higher glycosaminoglycan and collagen deposition.
The two first principal components accounted for 1.5%and0.7%7% of the genetic variation, whereas clearly separating the population isolates, like Sardinians, French Basques and northern Finns, but also the more homogenous FVG sub-populations.
We found this method produced a more highly correlated clustering structure when compared to other methods leading to more compact sub-clusters (Additional file 1).
