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In one case selected for illustration, this second nested PCR generated a more homogeneous template for DNA sequencing, resulting in a 72-base tracing with distinct base calling peaks for BLAST analysis.
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When a sample contained two or more genotypes of HPV DNA which were co-amplified during the first nested PCR and resulted in an ambiguous electropherogram with numerous overlapping base calling peaks, a second nested PCR could not generate a more homogeneous DNA template for DNA sequencing.
To investigate the presence of possible heterogeneity in Pol α, the average model obtained after refinement with EMAN (Ludtke et al, 1999) was used as the initial template for 3D maximum-likelihood (ML3D) classification (Scheres et al, 2007), to split the images into more homogeneous subsets of the data.
Surely because its team is more homogeneous.
But more cohesive, more homogeneous - can I use that word?
Superstitions disappear; cultures grow more homogeneous; life becomes increasingly rational.
"It's more homogeneous than other European nations".
Just as the world has become more homogeneous, so has the American academic calendar.
The genetic makeup of Ashkenazi Jews is more homogeneous than many other groups'.
It just adds to the impression that American fashion is becoming more homogeneous".
"They tend to be more homogeneous, and the vision of this church is to invite everybody".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com