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What's more, higher transfection efficiency was achieved.
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For comb-branched VGD polyplexes, VGD6 (G2) with higher molecular weight and more charges showed higher transfection efficiency in the presence of serum compared to VGD4 and VGD5 (G1).
We also tested wt and mutant BCL9 proteins in HEK 293 cells transiently stimulated with Wnt3A, since this provides a more sensitive assay (partly due to the higher transfection efficiency of these cells).
These lipids could give much higher transfection efficiency than those containing more flexible aliphatic linkage in various cell lines.
However, the transfection procedures were performed trying to minimize the cell loses, therefore it is also possible that part of the effect observed could be derived from a higher transfection efficiency of the cells that are more firmly attached versus the cells that are loosely attached or forming clusters.
More importantly, CPNA and PPNA were not sensitive to serum and showed the similar or higher transfection efficiency of pDNA and siRNA compared to Lipofectamine 2000.
An exception was VGD8, which despite being a G0 dendrimer exhibited considerably higher transfection, especially at N/P > 8. Following a similar trend, VGD9 and VGD10 (G1) with higher molecular weight and more charges performed better than G0 VGD.
In vitro experiments show that these polymers have lower cytotoxicity and higher transfection efficiency than 25 KDa PEI.
Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency.
In comparison with blank chitosan, CNT-chitosan nanocomplexes have exhibited significantly higher transfection efficiency [88].
Most of the polymers have higher transfection efficiency than 25 kDa PEI in the in vitro transfection experiments.
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