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Overall, significantly more genotypes were detected by SPF10 LiPA25 (kappa = 0.853, p = 0.022).
As more genotypes were tested, however, we found that the number of eggs laid was highly variable among genotypes,and that females of some lines never laid eggs in this assay.This variation could be due to many factors, but may in part reflect differences in acceptance of oviposition media betweenlines.
Three more genotypes were significantly differentiated from the core genotypes, but branch lengths were considerably shorter (IX, DAOM181602, XIV; Fig. 1).
Recently, two more genotypes were found: G, in North America and Europe [ 10] and H, in North and Central America [ 11].
In addition, 8 subjects with missing values for 2 or more genotypes were omitted in the analysis of DNMT1, DNMT3A, and DNMT3B genotypes and haplotypes and LINE-1 methylation index.
Because more genotypes were imputed when offspring were genotyped, there was also more chance that they were imputed incorrectly and, therefore, the percentage of incorrectly imputed SNPs was higher with genotyped offspring.
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Selfing must occur when only one parasite genotype is present in human blood and will also occur by chance even if two or more genotypes are present.
MOI is the result of two ecologically distinct processes that are hard to differentiate by genotyping only: coinfections (two or more genotypes being transmitted simultaneously by a mosquito) and superinfections (a patient acquiring multiple but independent infections).
In contrast to our results, George et al. [ 13] have reported that, if more genotypes are missing, the QTL variance is overestimated, the residual polygenic variance is underestimated and bias is increased.
Monoclonality was defined as the presence of only one allele of the three major msp1 types in the sample while isolates with two or more genotype were considered as polyclonal infection [ 30].
Meanwhile, it has become clear that the size of the reference population used for training is an important factor for the reliability of genomic breeding values [ 3, 4], which means that more genotyping is required.
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