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Moreover, those zones also appear to be functionally specialised with most proteins in a zone belonging to the top four enriched pathways, while the outer zones are much more functionally diversified (Table 9, Additional file 1: Table S1).
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On the other hand, the divergence in motif composition among different classes may indicate that they are functionally diversified.
The differences in these characteristics among the different clades suggested that the WRKY members were functionally diversified.
In many cases members of a gene family have become functionally diversified by changes in their expression patterns but their activities are still interchangeable.
Otx gene duplications are purported to have occurred in a gnathostome ancestor, and, subsequently, each paralogue has functionally diversified (Germot et al., 2001; Suda et al., 2009).
On the other hand, the differences between gene organization and the divergences in motif compositions among different subfamilies may also indicate that Populus NACs are functionally diversified.
Saprolegnia and Phytophthora species have developed, expanded and functionally diversified a subfamily of receptors that are structurally, but not phylogenetically, related to plant LRR-RLKs.
It has been proven that both MoDcl1 and MoDcl2 define functionally diversified RNAi pathways which may have arisen from transcriptional control and protein specialization [ 80].
On the one hand, NLRP genes duplicated and functionally diversified in mammalian reproductive systems (such as NLRP4, 5, 9 and 14).
In addition, the variation in gene structure and the difference in motif compositions among different subfamilies indicate that GAox subfamilies are functionally diversified.
Key components of the RNA-directed DNA methylation pathway include two functionally diversified RNA polymerase II (Pol II -related RNA polymerases called Pol II -relatedV [ 1].
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