By gradually increasing the antibiotic concentration in the medium, we force the cells to evolve in a way that allows more functional target-GFP ErmC target-GFP ErmC.
Then when one method gained more functional binding target sets of TFs than the other, we said that this method is more expression coherent than the other under this expression time series condition.
Interestingly, CD56dimNKG2A−KIR+ cells were more functional against 721.221 target cells expressing HLA-E than against their non-transfected counterparts (Fig. 5B and 5D), which suggest preferential expression of NKG2C on CD56dimNKG2A− cells.
Together these observations support that the hypothesis that repressed targets under the endogenous conditions are more likely to be functional targets.
While transient interaction of ITCs with some protein thiols (as well as with glutathione) likely occur in intact cells, stable covalent modifications of proteins are more likely to represent functional targets, particularly at low ITC concentration.
In target annotation, conservation scores could be applied to filter the less conserved targets, since functional targets are more likely to be conserved between different species.
Nonetheless, much remains to be performed both experimentally and bioinformatically for these and many more in pathway enrichment, identifying functional targets, regulatory relationships and protein protein interaction sub-networks to properly contextualise these entities and understand their contribution to prostate cancer progression.
Later, the acquisition of more functional domains, upstream regulators and downstream targets makes the Hippo pathway to evolve to become one of the most conserved and essential developmental toolkits for tissue and organ size control in Bilateria.
Our future study includes the expansion of our study objectives to generate more detailed second-generation or third-generation regulatory networks for Nrf2 and Nfkb1 as more functional data emerge on these gene targets of interest and their interactions with coactivator/corepressor modules that associate with them.
Once we have identified potential target genes, we can do more functional analyses in model organisms.
